Journal: Molecules

Article Title: In Vitro Antiglycation and Methylglyoxal Trapping Effect of Peppermint Leaf ( Mentha × piperita L.) and Its Polyphenols

doi: 10.3390/molecules28062865

Figure Lengend Snippet: Chemical profile of peppermint leaf dry extract confirmed by UHPLC-ESI-MS.

Article Snippet: Eriodictyol (CAS No. 552-58-9), luteolin (CAS No. 491-70-3), luteolin-7- O -β-glucoside (CAS No. 5373-11-5), apigenin-7- O -rutinoside (CAS No. 552-57-8, syn. isorhoifolin), naringenin-7- O -rutinoside (CAS No. 14259-46-2, syn. narirutin), hesperetin-7- O -rutinoside (CAS No. 520-26-3, syn. hesperidin), diosmetin-7- O -rutinoside (CAS No. 520-27-4, syn. diosmin), caffeic acid (CAS No. 331-39-5), and rosmarinic acid (CAS No. 20283-92-5) were purchased from Extrasynthese (Genay, France).

Techniques: Tandem Mass Spectroscopy

Journal: Molecules

Article Title: In Vitro Antiglycation and Methylglyoxal Trapping Effect of Peppermint Leaf ( Mentha × piperita L.) and Its Polyphenols

doi: 10.3390/molecules28062865

Figure Lengend Snippet: HPLC chromatograms of peppermint leaf dry extract (1.5 mg/mL, 50% aq. methanol); red at 280 nm, blue 320 nm, and black 360 nm. Peak labeling: 1, eriocitrin; 2, luteolin-7- O -rutinoside; 3, luteolin-7- O -β-glucuronoside; 4, narirutin; 5, isorhoifolin; 6, diosmin; 7, hesperidin; 8, lithospermic acid; 9, rosmarinic acid; 10, caffeic acid; 11, eriodictyol.

Article Snippet: Eriodictyol (CAS No. 552-58-9), luteolin (CAS No. 491-70-3), luteolin-7- O -β-glucoside (CAS No. 5373-11-5), apigenin-7- O -rutinoside (CAS No. 552-57-8, syn. isorhoifolin), naringenin-7- O -rutinoside (CAS No. 14259-46-2, syn. narirutin), hesperetin-7- O -rutinoside (CAS No. 520-26-3, syn. hesperidin), diosmetin-7- O -rutinoside (CAS No. 520-27-4, syn. diosmin), caffeic acid (CAS No. 331-39-5), and rosmarinic acid (CAS No. 20283-92-5) were purchased from Extrasynthese (Genay, France).

Techniques: Labeling

Journal: Molecules

Article Title: In Vitro Antiglycation and Methylglyoxal Trapping Effect of Peppermint Leaf ( Mentha × piperita L.) and Its Polyphenols

doi: 10.3390/molecules28062865

Figure Lengend Snippet: Content of key polyphenols in peppermint leaf dry extract determined by HPLC-DAD (as heat-map).

Article Snippet: Eriodictyol (CAS No. 552-58-9), luteolin (CAS No. 491-70-3), luteolin-7- O -β-glucoside (CAS No. 5373-11-5), apigenin-7- O -rutinoside (CAS No. 552-57-8, syn. isorhoifolin), naringenin-7- O -rutinoside (CAS No. 14259-46-2, syn. narirutin), hesperetin-7- O -rutinoside (CAS No. 520-26-3, syn. hesperidin), diosmetin-7- O -rutinoside (CAS No. 520-27-4, syn. diosmin), caffeic acid (CAS No. 331-39-5), and rosmarinic acid (CAS No. 20283-92-5) were purchased from Extrasynthese (Genay, France).

Techniques:

Journal: Molecules

Article Title: In Vitro Antiglycation and Methylglyoxal Trapping Effect of Peppermint Leaf ( Mentha × piperita L.) and Its Polyphenols

doi: 10.3390/molecules28062865

Figure Lengend Snippet: Antiglycation activity after seven-day incubation of bovine serum albumin with methylglyoxal (0.5 mM) and the test compound (1.5 mM) or peppermint leaf dry extract (3 mg/mL) expressed as % inhibition of MGO-induced AGE formation. Results are representative of three experiments performed as mean ± SD. Data were analyzed by one-way ANOVA ( p < 0.0001) followed by Tukey’s multiple comparisons test; values not sharing a common letter are significantly different. Abbreviations: AP, apigenin; LU, luteolin; LU-7GR, luteolin-7- O -β-glucuronoside; LU-7R, luteolin-7- O -rutinoside; LU-7G, luteolin-7- O -β-glucoside; HT, hesperetin; ED, eriodictyol; ED-7R, eriocitrin; RA, rosmarinic acid; DPE, peppermint leaf dry extract; MET, metformin.

Article Snippet: Eriodictyol (CAS No. 552-58-9), luteolin (CAS No. 491-70-3), luteolin-7- O -β-glucoside (CAS No. 5373-11-5), apigenin-7- O -rutinoside (CAS No. 552-57-8, syn. isorhoifolin), naringenin-7- O -rutinoside (CAS No. 14259-46-2, syn. narirutin), hesperetin-7- O -rutinoside (CAS No. 520-26-3, syn. hesperidin), diosmetin-7- O -rutinoside (CAS No. 520-27-4, syn. diosmin), caffeic acid (CAS No. 331-39-5), and rosmarinic acid (CAS No. 20283-92-5) were purchased from Extrasynthese (Genay, France).

Techniques: Activity Assay, Incubation, Inhibition

Journal: Molecules

Article Title: In Vitro Antiglycation and Methylglyoxal Trapping Effect of Peppermint Leaf ( Mentha × piperita L.) and Its Polyphenols

doi: 10.3390/molecules28062865

Figure Lengend Snippet: Adducts of methylglyoxal with selected standards and polyphenols identified in peppermint leaf dry extract.

Article Snippet: Eriodictyol (CAS No. 552-58-9), luteolin (CAS No. 491-70-3), luteolin-7- O -β-glucoside (CAS No. 5373-11-5), apigenin-7- O -rutinoside (CAS No. 552-57-8, syn. isorhoifolin), naringenin-7- O -rutinoside (CAS No. 14259-46-2, syn. narirutin), hesperetin-7- O -rutinoside (CAS No. 520-26-3, syn. hesperidin), diosmetin-7- O -rutinoside (CAS No. 520-27-4, syn. diosmin), caffeic acid (CAS No. 331-39-5), and rosmarinic acid (CAS No. 20283-92-5) were purchased from Extrasynthese (Genay, France).

Techniques:

Journal: Molecules

Article Title: In Vitro Antiglycation and Methylglyoxal Trapping Effect of Peppermint Leaf ( Mentha × piperita L.) and Its Polyphenols

doi: 10.3390/molecules28062865

Figure Lengend Snippet: Proposed hemiacetal structures of mono-MGO and di-MGO adducts of luteolin (R1, -OH), apigenin (R1, -H), eriodictyol (R2, -OH) and eriocitrin (R2, -O-rutinoside).

Article Snippet: Eriodictyol (CAS No. 552-58-9), luteolin (CAS No. 491-70-3), luteolin-7- O -β-glucoside (CAS No. 5373-11-5), apigenin-7- O -rutinoside (CAS No. 552-57-8, syn. isorhoifolin), naringenin-7- O -rutinoside (CAS No. 14259-46-2, syn. narirutin), hesperetin-7- O -rutinoside (CAS No. 520-26-3, syn. hesperidin), diosmetin-7- O -rutinoside (CAS No. 520-27-4, syn. diosmin), caffeic acid (CAS No. 331-39-5), and rosmarinic acid (CAS No. 20283-92-5) were purchased from Extrasynthese (Genay, France).

Techniques:

Journal: Molecular Metabolism

Article Title: The role of intestinal microbiota in physiologic and body compositional changes that accompany CLA-mediated weight loss in obese mice

doi: 10.1016/j.molmet.2024.102029

Figure Lengend Snippet: Energy balance and glucose metabolism in conventionally-raised mice given cecal transplants in Experiment 2. Male Ldlr −/− mice were fed a HFHS diet for 10 weeks, then given ABX-containing water for 1 week. One day after returning to normal water, mice were given cecal transplants from donor mice that been fed an HFHS diet (Ob-CT, n = 8) or an HFHS diet + 1% 10,12 CLA (CLA-CT, n = 8). Saline gavage was given to a control (SAL, n = 8). (A) Body weights. (B) Body composition at two time points. (C) EWAT, IWAT, and liver mass at sacrifice. (D) Fasting glucose at 2 time points: 10 weeks (B: baseline) and 15 weeks (C: post-CT). (E) Intraperitoneal glucose tolerance test (GTT, 1 mg/kg lean mass), GTT area under the curve (AUC, arbitrary units (A.U.)), and plasma insulin measured at the 30-minute time point. (F) Intraperitoneal insulin tolerance test (ITT). ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001; ns: not significant. Data were analyzed using one- and two-way ANOVA with Tukey's multiple comparison's test to compare the mean of each group to each other, presented as mean ± standard error.

Article Snippet: Group-housed mice were fed an HFHS diet (BioServ #F4997: 58.9% kcal from fat [lard], 26.2% kcal from sucrose, and 0.15% added cholesterol) ( ) [ ] for 10 weeks (baseline), then singly housed and divided into two groups: one received an antibiotic cocktail administered ad libitum in the drinking water (ABX: 1 g/L ampicillin, 1 g/L neomycin, 0.5 g/L vancomycin, and 0.5 g/L metronidazole with sugar-free marshmallow flavoring), while the other received vehicle-containing water (VEH: sugar-free marshmallow flavoring).

Techniques: Saline, Control, Comparison

Journal: Molecular Metabolism

Article Title: The role of intestinal microbiota in physiologic and body compositional changes that accompany CLA-mediated weight loss in obese mice

doi: 10.1016/j.molmet.2024.102029

Figure Lengend Snippet: Energy balance and glucose metabolism in GF-raised mice given cecal transplants in Experiment 3. Male C57BL/6J mice reared germ-free (GF) were inoculated with cecal contents from Ldlr −/− mice that had been fed a chow diet (Chow), an HFHS diet for 20 weeks (Ob), or an HFHS diet for 20 weeks + 1% 10,12 CLA given during the last 8 weeks (CLA). Following cecal transplant (CT), recipient mice were maintained on a normal chow diet for 4 weeks, then switched to a HFHS diet for an additional 5 weeks. (A) Body composition taken at 2 time points (weeks 9 and 14) in a subset of mice (n = 2–4). (B) Body weights after CT. ( C ) Liver, EWAT, and IWAT weight at sacrifice, as a percent of total body weight. ( D ) Fasting (4-hours) glucose levels. (E) Intraperitoneal glucose tolerance tests (GTT) performed at 9 weeks (GTT-1) and 13 weeks (GTT-2), with area under the curve (AUC, arbitrary units (A.U.)) calculated. (F) IWAT and liver mRNA expression for Adipoq (adiponectin), Lgals3 (galectin-3 or Mac2), Ucp1 (uncoupling protein 1), Pck1 (phosphoenolpyruvate carboxykinase 1, or Pepck), Slc2a2 (glucose transporter 2, or Glut2), Fasn (fatty acid synthase), Saa2 (serum amyloid A2), and Cpt1a (carnitine palmitoyltransferase 1 alpha). ∗p < 0.05; ∗∗p < 0.01; ∗∗∗∗p < 0.0001. Data were analyzed using one- and two-way ANOVA with Tukey's multiple comparison's test to compare the mean of each group to each other, presented as mean ± standard error.

Article Snippet: Group-housed mice were fed an HFHS diet (BioServ #F4997: 58.9% kcal from fat [lard], 26.2% kcal from sucrose, and 0.15% added cholesterol) ( ) [ ] for 10 weeks (baseline), then singly housed and divided into two groups: one received an antibiotic cocktail administered ad libitum in the drinking water (ABX: 1 g/L ampicillin, 1 g/L neomycin, 0.5 g/L vancomycin, and 0.5 g/L metronidazole with sugar-free marshmallow flavoring), while the other received vehicle-containing water (VEH: sugar-free marshmallow flavoring).

Techniques: Expressing, Comparison